Vasectomy Reversal Research

The Intussuscepted Vasovasostomy: A simple, novel technique

Aaron J. Milbank, Wayne Kuang and Anthony J. Thomas

Objective: Common contemporaneous methods for performing vasovasostomy include the two layer vasovasostomy and the modified one layer vasovasostomy, both of which require at least 12 sutures. The intussuscepted vasoepididymostomy is now an accepted and commonly used method for anastomosing the epididymis to the vas deferens. We postulated that the principle of intussusception could be adapted to vasovasostomy to create a widely patent, water-tight and simply crafted anastomosis.

Methods: We compared the two layer VV (2LVV) technique to the Intussusception VV (IVV) with respect to patency, leak and ease of performance as assessed by anastomotic time and number of haptic events. We performed 5 IVV’s and 5 2LVV’s on human vasa deferentia. The IVV is performed using 9-0 Nylon double armed suture (2.5 cm). The technique incorporates the principles of a horizontal mattress closure. The 1 st suture is placed with both needles passed full thickness from outside to inside the lumen on the left hand sided vas, one at 5:30 and one at 6:30 on a clock face. The other 2 sutures are placed in a similar fashion but at the 1:30/2:30 and 9:30/10:30 locations. All needles are passed from inside the lumen to outside the lumen on the right hand side of the vas deferens in matching positions. Three 9-0 nylon sutures (remnants) are then placed through the muscularis to further bolster the anastomosis. The 2LVV was performed as previously described. We recorded the anastomotic time, # sutures used, suture breakage, needle damage, patency as assessed by irrigation and suture passage, and presence or absence of leak. Following completion of the patency/leak tests, all specimens were opened to subjectively assess patency at the anastomosis and to determine if one technique produced wider anastomoses.

Results: Objectively, the average anastomotic time for the 2LVV and the IVV were 36 minutes and 20 minutes, respectively (p<0.001). A total of 3 sutures were used for each of the IVV’s. All 10 anastomoses were patent and no leaks were dependent. There were two haptic events (bent needles) both in the 2LVV group. The lumens of the IVV anastomoses appeared larger than the lumen of the same vas distant from the anastomosis. This was not observed in the 2LVV specimens where the lumen at the anastamosis was similar to the lumen in the remainder of the vas segment.

Discussion: This pilot study demonstrates that the intussuscepted vasovasostomy is a technically simple method for vasal approximation associated with a high ex-vivo patency rate. It appears that this technique, by incorporating the principle of the horizontal mattress, provides a lumen at the anastomosis that is larger than the lumen elsewhere in the vas. Definitive statements regarding the clinical superiority of this technique must await in vivo stuadies.